Photosystem II Repair Cycle in Faba Bean May Play a Role in Its Resistance to Botrytis fabae Infection

Chocolate spot, which is caused by the necrotrophic fungus Botrytis fabae, a major foliar disease occurring worldwide and dramatically reducing crop yields in faba bean (Vicia faba). Although chemical control of this an option, it has serious economic environmental drawbacks that make resistant cultivars more sensible choice. The molecular mechanisms behind defense against B. fabae are poorly understood. In work, we studied leave proteome two genotypes respond differently to order expand available knowledge on such mechanisms. For purpose, used two-dimensional gel electrophoresis (2DE) combination with Matrix-Assisted Laser Desorption/Ionization (MALDI-TOF/TOF). Univariate statistical analysis gels revealed 194 differential protein spots, 102 were identified mass spectrometry. Most spots belonged proteins energy primary metabolism, degradation, redox or response stress functional groups. MS results validated assays protease activity gels. Overall, they suggest may different PSII repair cycle mechanism chloroplast. differences resistance be result metabolic imbalance susceptible genotype efficient chloroplast detoxification system at early stages infection.